Human enteroids support robust replication of enteric and respiratory adenoviruses, revealing interferon sensitivity and a preference of respiratory serotypes for goblet cells.
Human enteroids provide a unified platform for culturing enteric viruses and uncovering aspects of adenovirus biology unattainable with standard cell lines.
Enteric adenoviruses are a significant cause of childhood gastroenteritis worldwide, yet our understanding of their unique biology is limited. Here we report robust replication of both prototype and clinical isolates of enteric and respiratory human adenoviruses in enteroids, a primary intestinal cell culture system. Recent studies have shown that other fastidious enteric viruses replicate in human enteroids. Therefore, human enteroids may provide a unified platform for culturing enteric viruses, potentially enabling isolation of a greater diversity of viruses from patients. Moreover, both the ability of interferon to restrict respiratory and enteric adenoviruses and a surprising preference of a respiratory serotype for goblet cells demonstrate the power of this culture system to uncover aspects of adenovirus biology that were previously unattainable with standard cell lines.
Holly et al. (Wed,) conducted a other in Adenovirus infection. Human enteroids was evaluated. Human enteroids support robust replication of enteric and respiratory adenoviruses, revealing interferon sensitivity and a preference of respiratory serotypes for goblet cells.