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We present ggsashimi, a command-line tool for the visualization of splicing events across multiple samples. Given a specified genomic region, ggsashimi creates sashimi plots for individual RNA-seq experiments as well as aggregated plots for groups of experiments, a feature unique to this software. Compared to the existing versions of programs generating sashimi plots, it uses popular bioinformatics file formats, it is annotation-independent, and allows the visualization of splicing events even for large genomic regions by scaling down the genomic segments between splice sites. ggsashimi is freely available at https://github.com/guigolab/ggsashimi. It is implemented in python, and internally generates R code for plotting.
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Diego Garrido-Martín
Universitat de Barcelona
Emilio Palumbo
Azienda Ospedaliera di Valtellina e Valchiavenna
Roderic Guigó
University College Dublin
PLoS Computational Biology
SHILAP Revista de lepidopterología
Universitat Pompeu Fabra
Centre for Genomic Regulation
Hospital del Mar Research Institute
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Garrido-Martín et al. (Fri,) studied this question.
synapsesocial.com/papers/69db1a7a0d8d6ef495a3cc0f — DOI: https://doi.org/10.1371/journal.pcbi.1006360