Practical guidelines for rigor and reproducibility in preclinical and clinical studies on cardioprotection

Adenosine diphosphate ANOVA Analysis of variance ATP Adenosine triphosphate CK Creatine kinase CK-MB Creatine kinase-muscle/brain CMR Cardiac magnetic resonance imaging CsA Cyclosporine A ECG Electrocardiogram EGTA Ethylene glycol-bis(-aminoethyl ether)-N,N,N',N'-tetraacetic acid FCCP Carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone FCS Fetal calf serum HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ICH International conference on harmonization IFM Interfibrillar mitochondria JC-1 Tetraethyl-benzimidazolyl-carbocyanine iodide KHB Krebs-Henseleit buffer LDH Lactate dehydrogenase LGE Late gadolinium enhancement LOCF Last observation carried forward MACCE Major adverse cardiac and cerebral events MOPS 3-(N-morpholino)propanesulfonic acid mPTP Mitochondrial permeability transition pore MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium PPCI Primary percutaneous coronary intervention RNA Ribonucleic acid ROS Reactive oxygen species SPECT Single-photon emission computed tomography SSM Subsarcolemmal mitochondria STEMI ST segment elevation myocardial infarction T2w-CMR T2-weighted cardiac magnetic resonance imaging TMRE Ethyl ester of tetramethylrhodamine TMRM Methyl ester of tetramethylrhodamine TTC 2,3,5-triphenyl tetrazolium chloride