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BACKGROUND: -alkane and percentage of emission. Furthermore, we explored the usage of nearest components and single calibrators for semi-quantifications. RESULTS: Any of the semi-quantification methods based on a standard produced similar or even identical results compared to quantification by a true-standard for a compound, except for the method based on standard addition. Each method beholds advantages and disadvantages regarding level of accuracy, experimental variability, acceptance and retrieved quantities. CONCLUSIONS: Our data shows that, except for the method of standard addition to the biological sample, the rest of the semi-quantification methods studied give highly similar statistical results. Any of the methodologies presented here can therefore be considered as valid for scent profiling. Regarding relative proportions of VOCs, the generation of calibration curves for each compound analysed is not necessary.
Ruiz-Hernández et al. (Thu,) studied this question.