Mavacamten induced dose-dependent force depression that was less pronounced in cMyBPC-deficient murine myocardium compared to wild-type, while normalizing cross-bridge behavior.
Does mavacamten alter cross-bridge behavior and force generation in cMyBPC-deficient murine myocardium?
Mavacamten-induced force depression is modulated by cMyBPC expression levels, suggesting its clinical use in hypertrophic cardiomyopathy may need optimization based on the underlying molecular trigger.
Background Recent studies suggest that mavacamten (Myk461), a small myosin‐binding molecule, decreases hypercontractility in myocardium expressing hypertrophic cardiomyopathy‐causing missense mutations in myosin heavy chain. However, the predominant feature of most mutations in cardiac myosin binding protein‐C ( cMyBPC ) that cause hypertrophic cardiomyopathy is reduced total cMyBPC expression, and the impact of Myk461 on cMyBPC ‐deficient myocardium is currently unknown. Methods and Results We measured the impact of Myk461 on steady‐state and dynamic cross‐bridge ( XB ) behavior in detergent‐skinned mouse wild‐type myocardium and myocardium lacking cMyBPC (knockout (KO)). KO myocardium exhibited hypercontractile XB behavior as indicated by significant accelerations in rates of XB detachment (k rel ) and recruitment (k df ) at submaximal Ca 2+ activations. Incubation of KO and wild‐type myocardium with Myk461 resulted in a dose‐dependent force depression, and this impact was more pronounced at low Ca 2+ activations. Interestingly, Myk461‐induced force depressions were less pronounced in KO myocardium, especially at low Ca 2+ activations, which may be because of increased acto‐myosin XB formation and potential disruption of super‐relaxed XB s in KO myocardium. Additionally, Myk461 slowed k rel in KO myocardium but not in wild‐type myocardium, indicating increased XB “ on ” time. Furthermore, the greater degree of Myk461‐induced slowing in k df and reduction in XB recruitment magnitude in KO myocardium normalized the XB behavior back to wild‐type levels. Conclusions This is the first study to demonstrate that Myk461‐induced force depressions are modulated by cMyBPC expression levels in the sarcomere, and emphasizes that clinical use of Myk461 may need to be optimized based on the molecular trigger that underlies the hypertrophic cardiomyopathy phenotype.
Mamidi et al. (Tue,) conducted a other in Hypertrophic cardiomyopathy (murine model). Mavacamten (Myk461) vs. Wild-type myocardium was evaluated on Steady-state and dynamic cross-bridge behavior. Mavacamten induced dose-dependent force depression that was less pronounced in cMyBPC-deficient murine myocardium compared to wild-type, while normalizing cross-bridge behavior.