April 1, 2019
View Full PaperRNAi in Mammalian Cells by siRNA Duplex Transfection
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Abstract
Long dsRNA cannot be used in most cultured mammalian cells because it triggers the interferon response, causing widespread changes in gene expression and apoptosis. This protocol describes a method for delivering into mammalian cells siRNA duplexes that are too short to elicit the sequence-nonspecific responses associated with long dsRNA.