The combination of Captopril and insulin markedly improved cardiac fibrosis and decreased apoptosis in diabetic rats, whereas Captopril alone had minimal effects.
Does captopril combined with insulin reduce myocardial fibrosis and apoptosis in a rat model of type 1 diabetes?
In a rat model of type 1 diabetes, the combination of captopril and insulin markedly improved cardiac fibrosis and apoptosis compared to captopril alone.
Background Cardiomyopathy is one of the common complications of diabetes mellitus.Aim The aim of the present study was to investigate the effects of Captopril on myocardial fibrosis in streptozotocin-induced diabetic rats (an animal model of type 1 diabetes mellitus).Materials and methods Forty albino rats were divided into five groups. Group I (control group), group II (untreated diabetic rats), group III (insulin-treated diabetic rats), group IV (Captopril-treated diabetic rats), and group V (insulin and Captopril-treated diabetic rats) were killed at 4 and 8 weeks, and the samples were collected for histological evaluation using hematoxylin and eosin, Masson trichrome, and immunohistochemical staining with anti-P53 for apoptosis, alpha smooth muscle actin (α-SMA) for collagen deposition.Results Treatment of streptozotocin-induced diabetic rats with insulin and Captopril (group V) showed marked decrease in myocardial injury and apoptosis, which was confirmed by marked decrease of sarcoplasmic p53 expression. Group V also showed decreased collagen deposition as confirmed by Masson trichrome staining and α-SMA expression. The results were better after 8 weeks compared with those after 4 weeks.Conclusion The administration of Captopril alone with diabetic rats minimally affected cardiac fibrosis, while the combination of Captopril and insulin markedly improved cardiac fibrosis.
Attia et al. (Mon,) conducted a other in Streptozotocin-induced diabetes mellitus (type 1 diabetes model) (n=40). Captopril and insulin vs. Captopril alone, insulin alone, untreated diabetic rats, and healthy controls was evaluated on Myocardial fibrosis, injury, and apoptosis (histological and immunohistochemical evaluation). The combination of Captopril and insulin markedly improved cardiac fibrosis and decreased apoptosis in diabetic rats, whereas Captopril alone had minimal effects.
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