In a rat model of metabolic syndrome, 24-week sucrose feeding reduced the phosphorylation status of ryanodine receptor 2 at Ser2814 by 46% compared to controls, contributing to calcium mishandling.
In a rat model of metabolic syndrome, impaired in situ activity of RyR2 and reduced SERCA pump activity contribute to calcium mishandling and cardiac dysfunction.
Absolute Event Rate: 0.54% vs 1%
p-value: p=<0.05
Metabolic syndrome (MetS) has become a global epidemic. MetS is a serious health problem because of its related cardiovascular complications, which include hypertension and delayed heart rate recovery after exercise. The molecular bases of cardiac dysfunction in MetS are still under scrutiny and may be related to anomalies in the activity and expression of key proteins involved in the cardiac excitation-contraction coupling (ECC). The cardiac Ca 2+ channel/ryanodine receptor (RyR2) participates in releasing Ca 2+ from internal stores and plays a key role in the modulation of ECC. We examined alterations in expression, phosphorylation status, Ca 2+ sensitivity, and in situ function (by measuring Ca 2+ sparks and Ca 2+ transients) of RyR2; alterations in these characteristics could help to explain the Ca 2+ handling disturbances in MetS cardiomyocytes. MetS was induced in rats by adding commercially refined sugar (30% sucrose) to their drinking water for 24 weeks. Cardiomyocytes of MetS rats displayed decreased Ca 2+ transient amplitude and cell contractility at all stimulation frequencies. Quiescent MetS cardiomyocytes showed a decrease in Ca 2+ spark frequency, amplitude, and spark-mediated Ca 2+ leak. The 3 H-ryanodine binding data showed that functionally active RyRs are significantly diminished in MetS heart microsomes; and exhibited rapid Ca 2+ -induced inactivation. The phosphorylation of corresponding Ser2814 (a preferential target for CaMKII) of the hRyR2 was significantly diminished. RyR2 protein expression and Ser2808 phosphorylation level were both unchanged. Further, we demonstrated that cardiomyocyte Ca 2+ mishandling was associated with reduced SERCA pump activity due to decreased Thr17-PLN phosphorylation, suggesting a downregulation of CaMKII in MetS hearts, though the SR Ca 2+ load remained unchanged. The reduction in the phosphorylation level of RyR2 at Ser2814 decreases RyR2 availability for activation during ECC. In conclusion, the impaired in situ activity of RyR2 may also account for the poor overall cardiac outcome reported in MetS patients; hence, the SERCA pump and RyR2 are both attractive potential targets for future therapies.
Fernández-Miranda et al. (Tue,) conducted a other in Metabolic syndrome (n=26). 30% sucrose in drinking water vs. Water and commercial rat chow was evaluated on Normalized pSer2814RyR2 (phosphorylation status of Ser2814) (p=<0.05). In a rat model of metabolic syndrome, 24-week sucrose feeding reduced the phosphorylation status of ryanodine receptor 2 at Ser2814 by 46% compared to controls, contributing to calcium mishandling.