getITD for FLT3-ITD-based MRD monitoring in AML

1 Subjects AML cell line HL-60), 2 FLT3 -ITD positive controls (AML cell lines MOLM-14 and PL-21) and diagnosis samples of 28 AML patients who previously tested FLT3 -ITD positive by PCR-and capillary electrophoresis-based fragment analysis (FA).To further evaluate our assay in the context of MRD monitoring of FLT3 -ITD positive AML, we sequenced 29 follow-up samples of 10 of these AML patients, five of whom achieved continuous complete remission and five of whom relapsed from the disease.For all patients, at least one sample from complete remission, following induction therapy, and at least one additional follow-up sample, either from continued complete remission or the time of relapse,