Neutrophil depletion or myeloid deficiency of Wnt5a diminished transverse aortic constriction-induced hypertrophy and inflammation, and preserved cardiac function in mice.
Does Wnt5a-mediated neutrophil recruitment contribute to pressure overload-induced cardiac dysfunction in mice?
Wnt5a-regulated neutrophil infiltration plays a critical role in pressure overload-induced heart failure, highlighting a potential therapeutic target for nonischemic cardiac pathology.
BACKGROUND: Although the complex roles of macrophages in myocardial injury are widely appreciated, the function of neutrophils in nonischemic cardiac pathology has received relatively little attention. METHODS: To examine the regulation and function of neutrophils in pressure overload-induced cardiac hypertrophy, mice underwent treatment with Ly6G antibody to deplete neutrophils and then were subjected to transverse aortic constriction. RESULTS: Neutrophil depletion diminished transverse aortic constriction-induced hypertrophy and inflammation and preserved cardiac function. Myeloid deficiency of Wnt5a, a noncanonical Wnt, suppressed neutrophil infiltration to the hearts of transverse aortic constriction-treated mice and produced a phenotype that was similar to the neutropenic conditions. Conversely, mice overexpressing Wnt5a in myeloid cells displayed greater hypertrophic growth, inflammation, and cardiac dysfunction. Neutrophil depletion reversed the Wnt5a overexpression-induced cardiac pathology and eliminated differences in cardiac parameters between wild-type and myeloid-specific Wnt5a transgenic mice. CONCLUSIONS: These findings reveal that Wnt5a-regulated neutrophil infiltration has a critical role in pressure overload-induced heart failure.
Wang et al. (Fri,) conducted a other in Pressure overload-induced cardiac hypertrophy. Neutrophil depletion (Ly6G antibody) and Wnt5a genetic modulation vs. Wild-type/control mice was evaluated on Cardiac hypertrophy, inflammation, and cardiac function. Neutrophil depletion or myeloid deficiency of Wnt5a diminished transverse aortic constriction-induced hypertrophy and inflammation, and preserved cardiac function in mice.