Six novel porcine circovirus type 2 strains detected in China originated from inter-genotypic recombination within the Rep gene between PCV2c and PCV2d strains.
Porcine circovirus type 2 (PCV2) has been causing huge economic losses in Chinese swine herds since it was first identified in China in 1999. Genotypes of PCV2 except for PCV2c coexist in swine herds in China, which may facilitate virus recombination. In the current study, six novel PCV2 strains were detected in China, and these strains shared high nucleotide similarity of the Rep gene with the PCV2c strain DK1987PMWSfree and high homology of the Cap gene with PCV2d. Genome sequence analysis revealed that the complete genomes of these strains were 1767 nucleotides (nt) in length and shared 99.8%-99.9% nucleotide identity with each other and 91.7%-98.7% with representative strains. Phylogenetic analysis, sequencing analysis, base-by-base comparisons and comprehensive recombination analysis demonstrated that these six strains originated from recombination within the Rep gene between PCV2c and PCV2d strains. Surprisingly, further investigation through theoretical recombination analysis of Chinese PCV2 GenBank sequences showed that these novel patterns of recombinant PCV2 strains have been generated since 2010. Collectively, our findings provide additional evidence of inter-genotypic recombination of PCV2.
Wei et al. (Thu,) conducted a other in Porcine circovirus type 2 (PCV2) infection (n=6). PCV2c and PCV2d recombination was evaluated on Genomic sequence and recombination analysis. Six novel porcine circovirus type 2 strains detected in China originated from inter-genotypic recombination within the Rep gene between PCV2c and PCV2d strains.