Purification of a human prostate specific antigen. 1979 classical article.

No AccessJournal of UrologyCLINICAL UROLOGY: Milestone in Urology1 Mar 2002PURIFICATION OF A HUMAN PROSTATE SPECIFIC ANTIGEN1 M.C. WANG, L.A. VALENZUELA, G.P. MURPHY, and T.M. CHU M.C. WANGM.C. WANG More articles by this author , L.A. VALENZUELAL.A. VALENZUELA More articles by this author , G.P. MURPHYG.P. MURPHY More articles by this author , and T.M. CHUT.M. CHU Send requests for reprints to Dr. T. M. Chu, Roswell Park Memorial Institute, Buffalo, New York 14263.Submitted for publication March 26, 1979; accepted June 7, 1979. More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)65270-6AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Rabbit antiserum raised against the crude extract of normal human prostatic tissue contained antibodies to a prostatic tissue-specific antigen as shown by immunoprecipitation techniques. Using this antiserum a prostate antigen was detected in normal, benign hypertrophic, and malignant prostatic tissues, but not in other human tissues. The prostate antigen was purified to homogeneity from prostatic tissues and showed a single protein band on analytical polyacrylamide gel electrophoresis and isoelectric focusing. This report thus presents the first demonstration of the purification of a prostate-specific antigen that does not represent prostatic acid phosphatase. Department of Diagnostic Immunology Research and Biochemistry, Roswell Park Memorial Institute, Buffalo, New York (Reprinted with permission from Invest. Urol, 17: 159–163, 1979) References 1 : Carcinoma of the prostate in elderly men: incidence, growth characteristics and clinical significance.. J. Urol.1969; 101: 602. Abstract, Google Scholar 2 : An “acid” phosphatase occurring in the serum of patients with metastasizing carcinoma of the prostate gland.. J. Clin. Invest.1938; 17: 473. Google Scholar 3 : Studies on the antigenic properties of prostate tissue I.. J. Urol.1960; 84: 134. Abstract, Google Scholar 4 : Tissue- and species-specific antigens of normal human prostatic tissue.. J. Immunol.1970; 104: 1329. Google Scholar 5 : Immunologic studies of normal, benign and malignant human prostatic tissue.. Cancer1972; 29: 1570. Google Scholar 6 : Investigation of specific antigens in prostate cancer.. Cancer Chemother. Rep.1975; 59: 105. Google Scholar 7 : Tissue specific and tumor specific antigens in human prostate.. Fed. Proc.1977; 36: 1254. Google Scholar 8 : Isolation of Wilm’s tumor antigens by chelation.. Clin. Chim. Acta1975; 61: 411. Google Scholar 9 : Enzyme markers in human prostatic carcinoma.. Cancer Treat. Rep.1977; 61: 193. Google Scholar 10 : Immunochemical detection of serum prostatic acid phosphatase. Methodology and clinical evaluation.. Invest. Urol.1978; 15: 319. Google Scholar 11 : A procedure to increase the sensitivity of staining by Coomassie brilliant blue R250-perchloric acid solution.. Anal. Biochem.1976; 75: 634. Google Scholar 12 : The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.. J. Biol. Chem.1969; 244: 4406. Google Scholar 13 Weeke B. General remarks on principles, equipment, reagents and procedures. In A Manual of Quantitative Immunoelectrophoresis. Methods and Applications, p. 15, N. H. Axelson, J. Krøll, and B. Weeke (Editors). Universitetsforlaget, Oslo, 1973. Google Scholar 14 : Protein measurement with the Folin phenol reagent.. J. Biol. Chem.1951; 193: 265. Google Scholar 15 : An improved acid phosphatase procedure.. Clin. Chim. Acta1966; 13: 264. Google Scholar 16 : Purification and characterization of a human prostate tissue-specific antigen.. Fed. Proc.1979; 38: 1069. Google Scholar © 2002 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 167Issue 3March 2002Page: 1226-1230 Advertisement Copyright accepted June 7, 1979. More articles by this author Expand All Advertisement PDF downloadLoading ...