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Abstract An analytical procedure originally developed for the determination of aflatoxins in cottonseed products has been modified for application to many agricultural materials. Aflatoxins are rapidly extracted free of lipid contamination with 70% acetone. Many interfering pigments are removed from the crude extract by precipitation as insoluble lead derivatives, transfer of aflatoxins into chloroform, and further purification of the chloroform extract with silica gel. The procedure was compared with four recently proposed methods for the analysis of peanut products. This technique is capable of detecting as little as 0.3 ppb of aflatoxin Bi in peanuts and peanut butters. Adequate recovery of added aflatoxins was obtained when this method was applied to numerous different agricultural products.
Pons et al. (1966) studied this question.