Transplantation of human ADSC-derived beige adipocytes increased whole-body energy expenditure and significantly reduced circulating glucose levels in hyperglycemic mice compared to ADSC controls.
Does the transplantation of human beige adipocytes increase energy expenditure and reduce body weight in recipient mice?
Human beige adipocytes generated from adipose-derived stem cells show promise as a cell therapy for metabolic diseases by increasing energy expenditure and reducing body weight in mice, and as a tool for drug discovery.
Human beige adipocytes (BAs) have potential utility for the development of therapeutics to treat diabetes and obesity-associated diseases. Although several reports have described the generation of beige adipocytes in vitro, their potential utility in cell therapy and drug discovery has not been reported. Here, we describe the generation of BAs from human adipose-derived stem/stromal cells (ADSCs) in serum-free medium with efficiencies >90%. Molecular profiling of beige adipocytes shows them to be similar to primary BAs isolated from human tissue. In vitro, beige adipocytes exhibit uncoupled mitochondrial respiration and cAMP-induced lipolytic activity. Following transplantation, BAs increase whole-body energy expenditure and oxygen consumption, while reducing body-weight in recipient mice. Finally, we show the therapeutic utility of BAs in a platform for high-throughput drug screening (HTS). These findings demonstrate the potential utility of BAs as a cell therapeutic and as a tool for the identification of drugs to treat metabolic diseases.
Singh et al. (Tue,) conducted a other in Metabolic diseases. Human ADSC-derived beige adipocytes vs. ADSCs was evaluated on In vivo glucose clearance and energy expenditure. Transplantation of human ADSC-derived beige adipocytes increased whole-body energy expenditure and significantly reduced circulating glucose levels in hyperglycemic mice compared to ADSC controls.