Human sapovirus was successfully propagated in vitro using human cell lines supplemented with bile acids, enabling the evaluation of viral replication, minimum infectious dose, and disinfection methods.
The establishment of an in vitro cell culture system for human sapoviruses using a human duodenum cell line and bile acids provides a fundamental tool for future research and development of infection control strategies.
-fold. We also detected double-stranded RNA, viral nonstructural and structural proteins in the cell cultures, and intact HuSaV particles. We confirmed the infectivity of progeny viruses released into the cell culture supernatants by passaging. These results indicate the successful growth of HuSaVs in vitro. Additionally, we determined the minimum infectious dose and tested the sensitivities of HuSaV GI.1 and GII.3 to heat and ultraviolet treatments. This system is inexpensive, scalable, and reproducible in different laboratories, and can be used to investigate mechanisms of HuSaV replication and to evaluate antivirals and/or disinfection methods for HuSaVs.
Takagi et al. (Mon,) conducted a other in Human sapovirus (HuSaV) infection. Human cell lines supplemented with bile acids was evaluated on Successful growth of HuSaVs in vitro. Human sapovirus was successfully propagated in vitro using human cell lines supplemented with bile acids, enabling the evaluation of viral replication, minimum infectious dose, and disinfection methods.