Key result
Bile acids enable successful in vitro propagation of human sapovirus to evaluate replication and disinfection.
The establishment of an in vitro cell culture system for human sapoviruses using a human duodenum cell line and bile acids provides a fundamental tool for future research and development of infection control strategies.
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May enable sapovirus antiviral and disinfection testing; leaves open clinical translation pending further validation.
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Takagi et al. (2020) studied Human sapovirus (HuSaV) infection. Human cell lines supplemented with bile acids was evaluated on Successful growth of HuSaVs in vitro. Human sapovirus was successfully propagated in vitro using human cell lines supplemented with bile acids, enabling the evaluation of viral replication, minimum infectious dose, and disinfection methods.
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