Key result
Human sapoviruses successfully replicated in differentiated human intestinal enteroids from the small intestine, with viral RNA levels increasing 0.5 to 1.8 log10-fold, requiring bile acids but independent of secretor status.
Human Intestinal Enteroids serve as a physiologically relevant model for Human Sapovirus replication, which requires bile acid and is restricted to the small intestine.
Answers are grounded in this paper and cite their sources.
HIEs enable HuSaV replication studies in vitro; leaves open in vivo validation before clinical translation.
and are known to allow the replication of other enteric viruses. Successful infection of HIEs with two strains belonging to different genotypes of the virus allowed discovery that the tropism of these HuSaVs is restricted to the small intestine, does not occur in the colon, and replication requires bile acid but is independent of the expression of histo-blood group antigens. Thus, HIEs represent a physiologically relevant model to further investigate HuSaV biology and a suitable platform for the future development of vaccines and antivirals.
No takes yet. Share an insight, caveat, or question.
Euller-Nicolas et al. (2023) studied Human sapovirus infection (in vitro). Human sapovirus (HuSaV) infection vs. Uninfected controls or heat-inactivated virus was evaluated on Viral replication (fold change in viral RNA levels). Human sapoviruses successfully replicated in differentiated human intestinal enteroids from the small intestine, with viral RNA levels increasing 0.5 to 1.8 log10-fold, requiring bile acids but independent of secretor status.
Synapse has enriched 10 related papers. Consider them for comparative context: