Ablation of Kvβ2 in mice suppressed myocardial blood flow and impaired cardiac performance during stress, whereas ablation of Kvβ1.1 preserved cardiac function and modestly elevated blood flow.
Kvβ proteins differentially regulate vascular tone and myocardial blood flow, with Kvβ2 promoting and Kvβ1.1 inhibiting oxygen-dependent vasodilation during heightened metabolic demand.
Rationale: Voltage-gated potassium (Kv) channels in vascular smooth muscle are essential for coupling myocardial blood flow (MBF) with the metabolic demand of the heart. These channels consist of a transmembrane pore domain that associates with auxiliary Kvβ (voltage-gated potassium channel β)1 and Kvβ2 proteins, which differentially regulate Kv function in excitable cells. Nonetheless, the physiological role of Kvβ proteins in regulating vascular tone and metabolic hyperemia in the heart remains unknown. Objective: To test the hypothesis that Kvβ proteins confer oxygen sensitivity to vascular tone and are required for regulating blood flow in the heart. Methods and Results: Mice lacking Kvβ2 subunits exhibited suppressed MBF, impaired cardiac contractile performance, and failed to maintain elevated arterial blood pressure in response to catecholamine-induced stress. In contrast, ablation of Kvβ1.1 reduced cardiac workload, modestly elevated MBF, and preserved cardiac function during stress compared with wild-type mice. Coronary arteries isolated from Kvβ2 −/− , but not Kvβ1.1 −/− , mice had severely blunted vasodilation to hypoxia when compared with arteries from wild-type mice. Moreover, vasodilation of small diameter coronary and mesenteric arteries due to L-lactate, a biochemical marker of reduced tissue oxygenation and anaerobic metabolism, was significantly attenuated in vessels isolated from Kvβ2 −/− mice. Inducible enhancement of the Kvβ1:Kvβ2 ratio in Kv1 channels of arterial smooth muscle abolished L-lactate-induced vasodilation and suppressed the relationship between MBF and cardiac workload. Conclusions: The Kvβ proteins differentially regulate vascular tone and MBF, whereby Kvβ2 promotes, and Kvβ1.1 inhibits oxygen-dependent vasodilation and augments blood flow upon heightened metabolic demand.
Ohanyan et al. (Wed,) conducted a other in Myocardial blood flow regulation. Ablation of Kvβ2 or Kvβ1.1 subunits vs. Wild-type mice was evaluated on Myocardial blood flow, cardiac contractile performance, and vasodilation. Ablation of Kvβ2 in mice suppressed myocardial blood flow and impaired cardiac performance during stress, whereas ablation of Kvβ1.1 preserved cardiac function and modestly elevated blood flow.