Incorporating CRISPR-Cas systems with nucleic acid amplification strategies enables amplified detection signals, enrichment of low-abundance targets, and development of highly sensitive diagnostics.
The combination of CRISPR-Cas technology with nucleic acid amplification strategies provides a powerful platform for the rapid, sensitive, and specific point-of-care detection of nucleic acids, proteins, and small molecules.
-cleavage activity of Cas12 and Cas13, and unwinding and nicking ability of Cas9. Integrating a CRISPR-Cas system after nucleic acid amplification improves detection specificity due to RNA-guided recognition of specific sequences of amplicons. Incorporating CRISPR-Cas before nucleic acid amplification enables enrichment of rare and low-abundance nucleic acid targets and depletion of unwanted abundant nucleic acids. Unwinding of dsDNA to ssDNA using CRISPR-Cas9 at a moderate temperature facilitates techniques for achieving isothermal exponential amplification of nucleic acids. A combination of CRISPR-Cas systems with functional nucleic acids (FNAs) and molecular translators enables the detection of non-nucleic acid targets, such as proteins, metal ions, and small molecules. Successful integrations of CRISPR technology with nucleic acid amplification techniques result in highly sensitive and rapid detection of SARS-CoV-2, the virus that causes the COVID-19 pandemic.
Feng et al. (Fri,) reported a review. CRISPR-Cas systems with nucleic acid amplification was evaluated. Incorporating CRISPR-Cas systems with nucleic acid amplification strategies enables amplified detection signals, enrichment of low-abundance targets, and development of highly sensitive diagnostics.
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