Multiparameter assessment of microfluidic thrombus formation at physiological temperature improved the detection of acquired platelet dysfunction.
Does operating a multiparameter microfluidics assay of thrombus formation at physiological temperature improve sensitivity to contraction and antiplatelet agents compared to room temperature?
Performing microfluidic thrombus formation assays at physiological temperature (37 °C) enhances sensitivity to antiplatelet agents and platelet dysfunction, potentially improving diagnostic utility.
INTRODUCTION: Current developments to assess qualitative and quantitative platelet traits in flowed whole-blood are based on microfluidic devices that mostly operate at room temperature. However, operation at physiological temperature (37 °C) may increase the assay's sensitivity, and facilitates the comparison to other platelet function tests of the diagnostic laboratory. MATERIALS AND METHODS: ). These modifications allowed rapid testing of control and patient blood samples at physiological temperature. RESULTS AND CONCLUSION: and ADP in the buildup of contracted thrombi under flow. Overall, this study underlined the advantage of multiparameter assessment of microfluidic thrombus formation in detecting an acquired platelet dysfunction, when operating at physiological temperature. This work may bring microfluidics tests closer to the diagnostic laboratory.
Herfs et al. (Mon,) conducted a other in Acquired platelet dysfunction. Multiparameter microfluidics assay at physiological temperature (37 °C) vs. Room temperature was evaluated on Detection of acquired platelet dysfunction. Multiparameter assessment of microfluidic thrombus formation at physiological temperature improved the detection of acquired platelet dysfunction.