Key points are not available for this paper at this time.
Exosomes are messengers for intercellular communication and signal transduction. Circular RNA (circRNA) abnormal expression and regulation are involved in the occurrence and development of a variety of tumors. In the present study, exosomes in the serum of five patients with non-small cell lung cancer (NSCLC) were isolated before and after EGFR-TKIs resistance, and the circRNA expression profile was screened using a circRNA microarray. The effects of the exosome circRNA₁02481 on cell proliferation and apoptosis were analyzed. The interaction between miR-30a-5p and circRNA₁02481 or ROR1 was predicted by starBase software, and was confirmed by RNA pull-down and dual-luciferase reporter assays. The results showed that exosomes containing circRNA₁02481 were significantly up-regulated in NSCLC with EGFR-TKIs resistance (pin vitro (p<0. 05). CircRNA₁02481 served as a miR-30a-5p sponge to regulate ROR1 expression (p<0. 05). Furthermore, the expression of circRNA₁02481 in exosomes was associated with TNM stage, tumor differentiation status, brain metastasis, and PFS and OS duration. Therefore, it was concluded that tumor-derived exosomal circRNA_ 102481 could contribute to EGFR-TKIs resistance via the microRNA-30a-5p/ROR1 axis in NSCLC. Exosomal circRNA₁02481 may serve as a novel diagnostic biomarker and a therapeutic target for EGFR-TKIs resistance in NSCLC.
Yang et al. (Wed,) studied this question.