The novel EV-D68-specific real-time PCR assay demonstrated significantly higher sensitivity than the existing WashU assay (100% versus 45%, P < 0.01) for detecting recently circulating EV-D68 strains.
Observational (n=189)
Do novel PCR assays improve detection sensitivity of Enterovirus D68 compared to existing assays?
Novel PCR assays demonstrate higher sensitivity for detecting recently circulating EV-D68 compared to existing assays.
Absolute Event Rate: 100% vs 45%
p-value: p=<0.01
= 135). In conclusion, our novel assays had higher sensitivity than the existing assay and might lead to more accurate diagnosis of recently circulating EV-D68. To prepare for future EV-D68 outbreaks, EV-D68-specific assays must be continuously monitored and updated.
Ikuse et al. (Wed,) conducted a observational in Enterovirus D68 infection (n=189). Novel EV-D68-specific real-time PCR (NU assay) vs. Washington University (WashU) real-time PCR assay was evaluated on Sensitivity for detecting EV-D68 in confirmed cases (p=<0.01). The novel EV-D68-specific real-time PCR assay demonstrated significantly higher sensitivity than the existing WashU assay (100% versus 45%, P < 0.01) for detecting recently circulating EV-D68 strains.