L-carnitine protected against cardiac and renal damage in an isoproterenol-induced myocardial infarction rat model by suppressing oxidative stress and inhibiting TNF-α and IL-1β.
Does L-carnitine protect against cardiac and renal damage in an isoproterenol-induced myocardial infarction rat model?
L-carnitine demonstrates cardioprotective and renoprotective effects in a rat model of isoproterenol-induced myocardial infarction by reducing oxidative stress and inflammation.
BRIEF INTRODUCTION: Myocardial infarction (MI) is a common manifestation of certain cardiac diseases where oxidative stress and fibrosis aggravate the condition markedly. MAIN OBJECTIVE OF THE STUDY: Investigation of L-carnitine's cardioprotective roles and mechanism of action in a rat model of MI. METHODS: To develop a MI animal model, Isoproterenol (ISO) was administered in male Long Evans rats where animals were divided into five groups (six rats/group). The oxidative stress and antioxidant enzyme activities were determined by different biochemical tests. The real-time PCR was performed to determine the expression of TNF-α and Il-1β. Histopathological observations by hematoxylin-eosin and Masson trichrome were made to observe the tissue damage and fibrosis in heart and kidney. SIGNIFICANT FINDINGS FROM THE STUDY: The ISO-treated rats showed increased levels of troponin I and lipid peroxidation and lower antioxidant enzyme activity in heart and kidney tissues. The levels of TNF-α and IL-1β were also increased in ISO-rats. Co-administration of L-carnitine with ISO reversed all these parameters. The elevated levels of uric acid and creatinine kinase and ALP, AST and ALT activities in ISO-rats were also significantly reduced by L-carnitine administration. L-carnitine markedly decreased the infiltration of inflammatory cells and improved the tissue architecture in heart and kidney. Control animals did not show any appreciable response upon L-carnitine administration. RELEVANT CONTRIBUTION TO KNOWLEDGE: These results suggest that L-carnitine plays a defensive role against cardiac and renal damage in ISO-treated MI rat model via suppressing oxidative stress and increasing antioxidant enzyme functions through inhibition of TNF-α and IL-1β.
Emran et al. (Tue,) conducted a other in Myocardial infarction (n=30). L-carnitine vs. Isoproterenol alone was evaluated on Cardiac and renal damage, oxidative stress, and inflammatory markers. L-carnitine protected against cardiac and renal damage in an isoproterenol-induced myocardial infarction rat model by suppressing oxidative stress and inhibiting TNF-α and IL-1β.