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migrate much more slowly than the ds -DNA, and a minute difference in migration distance caused by the conformation change of single strands is visualized by silver staining. Because single strands migrate slower, the area visualized is in the higher molecular weight range of gel electrophoresis. This requires longer electrophoresis times, frequently until BPB and XC run off the gel.
Kulinski et al. (Sat,) studied this question.
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