Elevation of miR-365 significantly prolonged action potential duration in Short-QT syndrome myocytes, whereas specific inhibition of miR-365 normalized prolonged action potentials in Long-QT syndrome.
miR-365 directly regulates key repolarizing ion channels in the human heart, and its modulation can normalize action potential duration in in vitro models of Long-QT and Short-QT syndromes.
. Finally, refractory period measurements in human myocardial slices substantiate the regulatory effect of miR-365 on action potential in adult human myocardial tissue. Our results delineate miR-365 to regulate human cardiac action potential duration by targeting key factors of cardiac repolarization.
Esfandyari et al. (Tue,) conducted a other in Short-QT syndrome and Long-QT syndrome (in vitro models). miR-365 manipulation (mimic-365 or antimiR-365) vs. Scrambled control oligonucleotides (mimic-Ctrl or antimiR-Ctrl) was evaluated on Action potential duration (cAPD90) and effective refractory period. Elevation of miR-365 significantly prolonged action potential duration in Short-QT syndrome myocytes, whereas specific inhibition of miR-365 normalized prolonged action potentials in Long-QT syndrome.
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