A newly developed electromechanical hiPSC-CM computer model generated a peak twitch tension of 0.44 kPa at 1 Hz pacing, which agrees with experimentally observed values (0.21-6.5 kPa).
The newly developed electromechanical hiPSC-CM computer model successfully replicates experimental active tension, calcium transient, and action potential biomarkers, enabling future in silico cardiology studies.
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) enable accessible human-based cardiology studies. However, an immature hiPSC-CM electrophysiological and contractile phenotype hinders data translation to adult cardiomyocytes. In silico hiPSC-CM investigations could aid in hiPSC-CM data translation but most hiPSC-CM models do not feature a contractile element which limits their application for such studies. To address this issue, we have developed an electromechanical hiPSC-CM computer model by coupling an electrophysiological hiPSC-CM model and a human contractile machinery model. The newly established model has been calibrated using experimental hiPSC-CM data. We demonstrate that the computed active tension, calcium transient and action potential biomarkers agree with the experimental ranges. The peak twitch tension generated at 1 Hz pacing was 0.44 kPa which is in range with experimentally observed values (0.21-6.5 kPa). Comparisons with the adult myocyte electromechanical model demonstrate the potential usability of the hiPSC-CM model in the future data translation. Altogether, we present a new electromechanical hiPSC-CM model for comprehensive in silico hiPSC-CM-based studies.
Folkmanaite et al. (Mon,) conducted a other in hiPSC-CM electro-mechanical modeling. Electromechanical hiPSC-CM computer model vs. Experimental hiPSC-CM data and adult myocyte model was evaluated on Active tension, calcium transient and action potential biomarkers. A newly developed electromechanical hiPSC-CM computer model generated a peak twitch tension of 0.44 kPa at 1 Hz pacing, which agrees with experimentally observed values (0.21-6.5 kPa).