Long-term cultivation of human atrial myocardium preserved viability and electromechanical function, with no significant difference in contraction force between day 0 (384 ± 69 µN) and day 12 (255 ± 90 µN).
Absolute Event Rate: 255% vs 384%
p-value: p=0.8
Organotypic culture of human ventricular myocardium is emerging in basic and translational cardiac research. However, few institutions have access to human ventricular tissue, whereas atrial tissue is more commonly available and important for studying atrial physiology. This study presents a method for long-term cultivation of beating human atrial myocardium. After written informed consent, tissues from the right-atrial appendage were obtained from patients with sinus rhythm undergoing open heart surgery with cardiopulmonary bypass. Trabeculae (pectinate muscles) prepared from the samples were installed into cultivation chambers at 37°C with a diastolic preload of 500 μN. After 2 days with 0.5 Hz pacing, stimulation frequency was set to 1 Hz. Contractile force was monitored continuously. Beta-adrenergic response, refractory period (RP) and maximum captured frequency (f max ) were assessed periodically. After cultivation, viability and electromechanical function were investigated, as well as the expression of several genes important for intracellular Ca 2+ cycling and electrophysiology. Tissue microstructure was analyzed by confocal microscopy. We cultivated 19 constantly beating trabeculae from 8 patient samples for 12 days and 4 trabeculae from 3 specimen for 21 days. Functional parameters were compared directly after installation (0 d) with those after 12 d in culture. Contraction force was 384 ± 69 μN at 0 d and 255 ± 90 μN at 12 d ( p = 0.8, n = 22), RP 480 ± 97 ms and 408 ± 78 ms ( p = 0.3, n = 9), f max 3.0 ± 0.5 Hz and 3.8 ± 0.5 Hz ( p = 0.18, n = 9), respectively. Application of 100 nM isoprenaline to 11 trabeculae at 7 d increased contraction force from 168 ± 35 μN to 361 ± 60 μN ( p 0.01), f max from 6.4 ± 0.6 Hz to 8.5 ± 0.4 Hz ( p 0.01) and lowered RP from 319 ± 22 ms to 223 ± 15 ms. CACNA1c (L-type Ca 2+ channel subunit) and GJA1 (connexin-43) mRNA expressions were not significantly altered at 12 d vs 0 d, while ATP2A (SERCA) and KCNJ4 (Kir2.3) were downregulated, and KCNJ2 (Kir2.1) was upregulated. Simultaneous Ca 2+ imaging and force recording showed preserved excitation-contraction coupling in cultivated trabeculae. Confocal microscopy indicated preserved cardiomyocyte structure, unaltered amounts of extracellular matrix and gap junctions. MTT assays confirmed viability at 12 d. We established a workflow that allows for stable cultivation and functional analysis of beating human atrial myocardium for up to 3 weeks. This method may lead to novel insights into the physiology and pathophysiology of human atrial myocardium.
Klumm et al. (Wed,) conducted a other in Sinus rhythm patients undergoing open heart surgery (n=11). Long-term in vitro cultivation vs. Freshly isolated tissue (0 days) was evaluated on Contraction force (p=0.8). Long-term cultivation of human atrial myocardium preserved viability and electromechanical function, with no significant difference in contraction force between day 0 (384 ± 69 µN) and day 12 (255 ± 90 µN).