December 1, 1992
View Full PaperEpidemiology and prevention of blood pressure-related renal disease
Key Result
Platelets from dogs with essential hypertension showed significantly increased aggregation and secretion in response to ADP stimulation compared to normotensive controls.
Structured PICO
P
Population
Siberian husky dogs with hereditary essential hypertension and normotensive control dogs
I
Intervention
In vitro platelet stimulation with ADP, platelet-activating factor, thrombin, calcium ionophore A23187, or phorbol myristate acetate
C
Comparator
Platelets from normotensive control dogs
O
Outcome
Platelet aggregation and dense granule ATP secretionsurrogate
Siberian husky dogs with hereditary essential hypertension exhibit ADP-induced platelet aggregation defects similar to those seen in humans, suggesting utility as a preclinical model.
Abstract
Aim: Since canine hereditary essential hypertension has been previously reported in a colony of Siberian husky dogs, we tested the suitability of this model for use in studies on the platelet defect identified in humans with essential hypertension. Methods: Platelet aggregation and dense granule ATP secretion were measured in dogs with essential hypertension and normotensive control dogs. Results: The platelets from the hypertensive group showed significantly increased aggregation and secretion in response to stimulation with ADP. There was no significant increase in aggregation or secretion when platelets from hypertensive dogs were stimulated by platelet-activating factor, thrombin, calcium ionophore A23187 or phorbol myristate acetate. Conclusion: The increased aggregation and secretion responses in platelets from the hypertensive dogs suggest that a defect similar to that reported in humans with essential hypertension exists and that further investigation is warranted.