Severe hyperprolactinemia exacerbated high-fat diet-induced body weight gain and glucose intolerance, and promoted brown adipose tissue whitening with decreased thermogenic markers in female mice.
Does severe hyperprolactinemia aggravate High Fat Diet-induced metabolic imbalance and promote brown adipose tissue whitening in mice?
Severe hyperprolactinemia exacerbates high-fat diet-induced metabolic disturbances and promotes brown adipose tissue whitening in mice, suggesting prolactin may be an adjuvant factor in diet-induced obesity.
p-value: p=<0.05
Background The association of high serum prolactin and increased body weight is positive but controversial, therefore we hypothesized that additional factors such as diets and the impact of prolactin on brown adipose tissue may condition its metabolic effects. Methods We used LacDrd2KO females with lifelong severe hyperprolactinemia due dopamine-D2 receptor deletion from lactotropes, and slow onset of metabolic disturbances, and compared them to their respective controls (Drd2 loxP/loxP ). Food intake, and binge eating was evaluated. We then challenged mice with a High Fat (HFD) or a Control Diet (CD) for 8 weeks, beginning at 3 months of age, when no differences in body weight are found between genotypes. At the end of the protocol brown and white adipose tissues were weighed, and thermogenic and lipogenic markers studied, using real time PCR ( Ucp1, Cidea, Pgc1a, Lpl, adiponectin, Prlr ) or immunohistochemistry (UCP1). Histochemical analysis of brown adipose tissue, and glucose tolerance tests were performed. Results Hyperprolactinemic mice had increased food intake and binge eating behavior. Metabolic effects induced by a HFD were exacerbated in lacDrd2KO mice. Hyperprolactinemia aggravated HFD-induced body weight gain and glucose intolerance. In brown adipose tissue pronounced cellular whitening as well as decreased expression of the thermogenic markers Ucp1 and Pgc1a were observed in response to high prolactin levels, regardless of the diet, and furthermore, hyperprolactinemia potentiated the decrease in Cidea mRNA expression induced by HFD. In subcutaneous white adipose tissue hyperprolactinemia synergistically increased tissue weight, while decreasing Prlr , Adiponectin and Lpl mRNA levels regardless of the diet. Conclusions Pathological hyperprolactinemia has a strong impact in brown adipose tissue, lowering thermogenic markers and evoking tissue whitening. Furthermore, it modifies lipogenic markers in subcutaneous white adipose, and aggravates HFD-induced glucose intolerance and Cidea decrease. Therefore, severe high prolactin levels may target BAT function, and furthermore represent an adjuvant player in the development of obesity induced by high fat diets.
Lopez‐Vicchi et al. (Fri,) conducted a other in Hyperprolactinemia and High Fat Diet induced metabolic imbalance (n=22). High Fat Diet (HFD) and severe hyperprolactinemia vs. Control Diet (CD) and wild-type mice was evaluated on Brown adipose tissue whitening and thermogenic marker expression (p=<0.05). Severe hyperprolactinemia exacerbated high-fat diet-induced body weight gain and glucose intolerance, and promoted brown adipose tissue whitening with decreased thermogenic markers in female mice.