The expressions of KCNA5, Cx43, and Cx40 were substantially downregulated in the myocardium of patients with atrial fibrillation compared to those with sinus rhythm.
Case-Control
Are the expression levels and interactions of KCNA5, Cx43, and Cx40 altered in the left atrial appendage of patients with persistent atrial fibrillation compared to sinus rhythm?
The downregulation and interaction of KCNA5, Cx43, and Cx40 in the atrial myocardium may play a role in the molecular pathogenesis of persistent atrial fibrillation.
The objective of the study was to investigate the expression levels of potassium voltage-gated channel subfamily A member 5 (KCNA5), connexin 43 (Cx43), and connexin 40 (Cx40) in the left atrial appendage of patients with atrial fibrillation (AF) and the interactions between them. We gathered tissue samples from patients with persistent AF and sinus rhythm and used fluorescence quantitative polymerase chain reaction to evaluate messenger RNA (mRNA) changes of KCNA5, Cx43, and Cx40. Then, we studied the protein levels of KCNA5, Cx43, and Cx40 by immunofluorescence and western blot analysis and the interactions between these proteins were identified by immunoprecipitation and immunofluorescence colocation, respectively. Compared with the control group, the mRNA and protein levels of KCNA5, Cx43, and Cx40 in the AF group were decreased and the positive expression of KCNA5, Cx43, and Cx40 protein was also decreased by immunofluorescence staining in the AF group. In addition, immunoprecipitation and immunofluorescence colocation revealed that KCNA5 was coexpressed with Cx43 and Cx40 proteins. The expressions of KCNA5, Cx43, and Cx40 were substantially downregulated in the myocardium of patients with AF and KCNA5 interacted with Cx43 and Cx40 proteins, respectively.
Wang et al. (Sat,) conducted a case-control in Atrial fibrillation. Atrial fibrillation vs. Sinus rhythm was evaluated on mRNA and protein levels of KCNA5, Cx43, and Cx40. The expressions of KCNA5, Cx43, and Cx40 were substantially downregulated in the myocardium of patients with atrial fibrillation compared to those with sinus rhythm.
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