Culturing human induced pluripotent stem-cell-derived cardiomyocytes on a cardiac mimetic matrix accelerated their maturation within 30 days, enhancing metabolic and electrophysiological features.
Does a cardiac mimetic matrix accelerate the maturation of human induced pluripotent stem-cell-derived cardiomyocytes?
A cardiac mimetic matrix accelerates the maturation of hiPSC-CMs into adult-like ventricular myocytes, providing an improved model for preclinical cardiac research and drug screening.
The vast potential of human induced pluripotent stem-cell-derived cardiomyocytes (hiPSC-CMs) in preclinical models of cardiac pathologies, precision medicine, and drug screening remains to be fully realized because hiPSC-CMs are immature without adult-like characteristics. Here, we present a method to accelerate hiPSC-CM maturation on a substrate, cardiac mimetic matrix (CMM), mimicking adult human heart matrix ligand chemistry, rigidity, and submicron ultrastructure, which synergistically mature hiPSC-CMs rapidly within 30 days. hiPSC-CMs matured on CMM exhibit systemic transcriptomic maturation toward an adult heart state, are aligned with high strain energy, metabolically rely on oxidative phosphorylation and fatty acid oxidation, and display enhanced redox handling capability, efficient calcium handling, and electrophysiological features of ventricular myocytes. Endothelin-1-induced pathological hypertrophy is mitigated on CMM, highlighting the role of a native cardiac microenvironment in withstanding hypertrophy progression. CMM is a convenient model for accelerated development of ventricular myocytes manifesting highly specialized cardiac-specific functions.
Afzal et al. (Fri,) conducted a other in hiPSC-CM maturation. Cardiac mimetic matrix (CMM) was evaluated on hiPSC-CM maturation. Culturing human induced pluripotent stem-cell-derived cardiomyocytes on a cardiac mimetic matrix accelerated their maturation within 30 days, enhancing metabolic and electrophysiological features.