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Lysosomes are organelles with many functions in cell metabolism, the morphology changes as a function of the different cellular mechanisms. Some enzymes in the lysosomal medium are responsible for the cellular digestion process. This work reports a new biocompatible lysosome, the tracker, with the long-life fluorescence emission. They were investigated by steady-state, time-resolved spectroscopic, and confocal microscopy analysis in organic and biological mediums. It was observed that the probe could already be used at low concentrations to monitor lysosomes in live and fixed cell culture with selective fluorescence detection with high photostable spectroscopic. Therefore, a responsive emission in the red region of the light spectrum and the high fluorescence signal were observed even after 24 hours. The incubation time with highly selective lysosomal tracking and good response for long-time in vitro experiments, high solubility in a biological media, and reproducible signal response, with lower signal noise rates.
Barbosa et al. (Sat,) studied this question.