Temporal depletion of CD4+ T-cells during chronic heart failure significantly blunted left ventricular remodeling and prevented progressive cardiac dysfunction in mice.
Does temporal depletion of CD4+ T-cells during chronic heart failure improve left ventricular remodeling and cardiac function in a mouse model of myocardial infarction?
Temporal depletion of CD4+ T-cells during chronic ischemic heart failure blunts progressive left ventricular remodeling and cardiac dysfunction in a mouse model.
p-value: p=<0.05
CD4 + T-cells facilitate wound healing post-myocardial infarction (MI) but promote left-ventricular (LV) remodeling during ischemic heart failure (HF; 8 weeks post-MI). Therefore, it is critical to understand if sustained CD4 + T-cell activation leads to this pathological response, or if phenotypically different T-cells are activated during MI vs. HF. Using flow cytometry, we found that cardiac CD4 + T-cells exhibit two distinct patterns of transmigration. First pattern consisted of a rapid CD4 + T-cell response with maximal levels seen at 3 days post-MI which return to baseline by 14 days. However, during HF we observed a 2nd phase of activation and CD4 + T-cells were ∼20-fold higher in HF as compared to sham-operated mice. Importantly, these biphasic kinetics were observed with all major T-cell subsets such as Th1, Th2, Th17, and regulatory T-cells suggesting a global change. To determine the role of this 2nd peak of T-cell activation, CD4-iDTR mice were generated and treated with DT every 10 from 28 days post-MI to deplete CD4 + T-cells during chronic HF. While littermate control mice showed increased end-systolic and end-diastolic volumes (ESV and EDV) and decreased ejection fraction (EF) from 4 to 8 weeks post-MI, depletion of CD4 + T-cells in Cre + mice significantly blunted LV remodeling and inhibited progressive increases in the EDV and ESV, and reduction in EF. This suggests that CD4 + T-cell responses occurring during HF are different than those occurring during MI and promote LV remodeling and progressive cardiac dysfunction. Temporal immunomodulation of CD4 + T-cells could be a translatable modality for ischemic HF.
Kumar et al. (Thu,) conducted a other in Myocardial infarction and heart failure. CD4+ T-cell depletion (Diphtheria Toxin) vs. Littermate controls (Cre- mice) receiving DT was evaluated on Left ventricular remodeling (changes in EDV, ESV, and EF from 4 to 8 weeks post-MI) (p=<0.05). Temporal depletion of CD4+ T-cells during chronic heart failure significantly blunted left ventricular remodeling and prevented progressive cardiac dysfunction in mice.