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This study aims to investigate the development and the differences in student misconceptions in science based on gender and grade level, and to evaluate the developed two-tier multiple-choice diagnostic test in confirming the test's validity and reliability. A sample of 856 participants from 10th-12th graders and prospective science teachers were collected. The two-tier multiple-choice diagnostic test with 32 items covering biology, chemistry, and physics was administered to evaluate students' science misconceptions at the senior high school and university levels. The results met validity and reliability criteria using confirmatory factor analysis and Rasch parameters. The single-factor model has CFI = .973, RMSEA = .006, CI (.001, .014) and SRMR = .017 and the three-factor model has CFI = 0.939, RMSEA = .010, CI (.01, .017) and SRMR = .017. Based on the Rasch parameter, the infit and outfit MNSQ values achieve the acceptable fit (0.96 to 1) with good item reliability (.99) and person reliability (.80). All items have positive PTMA. Student misconceptions had significant differences in terms of grade and gender. We confirmed that prospective science teachers have higher misconceptions than 11th and 12th graders and slightly higher ones than 10th graders. Boys have a better conceptual understanding than girls based on the mean of correct answers. The multiple linear regression with the stepwise method confirmed that gender significantly predicted student misconceptions of science concepts, with 9% of variance explained. This study provided evidence that students and prospective teachers experience various misconceptions about science concepts.
Soeharto et al. (Thu,) studied this question.