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Evaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in defining correlates of protection. We describe an assay that uses single-cycle vesicular stomatitis virus (VSV) pseudoviruses linking a fluorophore with a spike (S) from a variant of concern (VOC). Using two fluorophores linked to two VOC S, respectively, allows us to determine the neutralization titer against two VOCs in a single run. This is a generalizable approach that saves time, samples, and run-to-run variability. For complete details on the use and execution of this protocol, please refer to Sievers et al. (2022).1.
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Benjamín L. Sievers
Terri Gelbart
Gene S. Tan
STAR Protocols
SHILAP Revista de lepidopterología
J. Craig Venter Institute
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Sievers et al. (Wed,) studied this question.
www.synapsesocial.com/papers/69dca0204006ebb8efe52e61 — DOI: https://doi.org/10.1016/j.xpro.2022.101835