Overexpression of the stress-enhanced cardiac lncRNA Morrbid significantly reduced myocardial infarct size and improved cardiac function in mice after acute myocardial infarction.
Does Morrbid overexpression reduce myocardial infarct size and improve cardiac function in a mouse model of acute myocardial infarction?
The stress-enhanced lncRNA Morrbid protects against acute myocardial infarction by reducing cardiomyocyte apoptosis via its target gene serpine1, highlighting a potential therapeutic target for ischemic heart disease.
p-value: p=<0.001
Myeloid RNA regulator of Bim-induced death (Morrbid) is a newly identified leukocyte-specific long noncoding RNA (lncRNA). However, the expression and biological functions of Morrbid in cardiomyocytes and heart disease are currently unclear. This study was meant to determine the role of cardiac Morrbid in acute myocardial infarction (AMI) and to identify the potential cellular and molecular mechanisms involved. We found that both human and mouse cardiomyocytes could express a significant amount of Morrbid and that its expression was increased in cardiomyocytes with hypoxia or oxidative stress as well as in mouse hearts with AMI. Overexpression of Morrbid reduced the myocardial infarct size and cardiac dysfunction, whereas the infarct size and cardiac dysfunction deteriorated in cardiomyocyte-specific Morrbid-KO (Morrbidfl/fl/Myh6-Cre) mice. We identified that Morrbid had a protective effect against hypoxia- or H2O2-induced apoptosis; this was also confirmed in vivo in mouse hearts after AMI. We further discovered that serpine1 was a direct target gene of Morrbid that was involved in the Morrbid-mediated protective effect on cardiomyocytes. In summary, we have found, for the first time to our knowledge, that the cardiac Morrbid is a stress-enhanced lncRNA that protects hearts from AMI via antiapoptosis through its target gene serpine1. Morrbid may be a novel promising therapeutic target for ischemic heart diseases such as AMI.
Yu et al. (Tue,) conducted a other in Acute myocardial infarction. Morrbid overexpression (Ad-Morrbid) vs. Ad-GFP was evaluated on Myocardial infarct size (p=<0.001). Overexpression of the stress-enhanced cardiac lncRNA Morrbid significantly reduced myocardial infarct size and improved cardiac function in mice after acute myocardial infarction.