Chronic lactate exposure in AC16 cardiomyocytes for 72 hours induced changes in several proteins and metabolites linked to cell hypertrophy and cytoskeleton remodelling.
Chronic lactate exposure in human cardiomyocytes induces proteomic and metabolomic changes associated with cell hypertrophy and cytoskeleton remodeling, potentially explaining cardiac adaptations in high-performance athletes.
We investigated the effect of growing on lactate instead of glucose in human cardiomyocyte assessing their viability, cell cycle activity, oxidative stress and metabolism by a proteomic and metabolomic approach. In previous studies performed on elite players, we found that adaptation to exercise is characterized by a chronic high plasma level of lactate. Lactate is considered not only an energy source but also a signalling molecule and is referred as "lactormone"; heart is one of the major recipients of exogenous lactate. With this in mind, we used a cardiac cell line AC16 to characterize the lactate metabolic profile and investigate the metabolic flexibility of the heart. Interestingly, our data indicated that cardiomyocytes grown on lactate (72 h) show change in several proteins and metabolites linked to cell hypertrophy and cytoskeleton remodelling. The obtained results could help to understand the effect of this metabolite on heart of high-performance athletes.
Luti et al. (Mon,) conducted a other in Cardiac cell line AC16. Lactate vs. Glucose was evaluated on Viability, cell cycle activity, oxidative stress and metabolism (proteomic and metabolomic profile). Chronic lactate exposure in AC16 cardiomyocytes for 72 hours induced changes in several proteins and metabolites linked to cell hypertrophy and cytoskeleton remodelling.