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Two γ-aminobutyric acid (GABA) producing Lactococcus spp.strains (K1180 and K1296) were isolated from kimchi.When incubated in MRS containing 1% monosodium glutamate at 30°C, both strains steadily increased GABA production from 24 to 48 hours.An antibiotic resistance evaluation of the two strains to determine their potential as probiotics showed that they met the safety criteria set by the European Food Safety Authority and were highly susceptible to all drugs investigated.Both strains lacked erythrocyte hemolytic activity, produced no biogenic amines, and displayed antibacterial activity against Klebsiella pneumoniae.In addition, both exhibited high survival rates of ca.90% in artificial gastric juice at pH 3.0 and of ca.50% in artificial bile salts at 0.5%.Notably, both strains also achieved adherences of >95% to intestinal epithelial cells.These findings confirm the probiotic potentials of Lactococcus lactis K1180 and K1296.
Ahn et al. (Thu,) studied this question.