eRNA extraction from water samples filtered through 47 mm diameter filters (NucleoMag DNA/RNA Water Kit - MACHEREY NAGEL). v1

The objective of this protocol is the environmental RNA (eRNA) extraction from water samples filtered through 47 mm diameter filters. RNA extraction is performed using a MagnetaPure 32 Nucleic Acid Purification System (Dutscher) and with the NucleoMagDNA/RNA Water Kit (Macherey Nagel), with a rDNase digestion step. The procedure is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. The benefits of using the MagnetaPure 32 Nucleic Acid Purification System (Dutscher) are increased productivity and repeatability, as well as eliminating human error and the pain of repetitive work. This protocol is used prior to molecular biology analysis (e.g. qPCR, metabarcoding, ddPCR) to specifically target both macro- and micro-organisms eRNA extracted from water samples.