Fecal biomarkers in Italian anthropogenic soil horizons and deposits from Middle Ages and bronze age

Archaeological excavations in urban and rural contexts often uncover dark homogeneous anthropogenic deposits, soils and soil horizons, known as Dark Earths, Cultural Layers and Anthrosols. Major scientific questions arise about the processes that lead to the formation of these soils and deposits, as they are often related to a complex combination of environmental, climatic, and anthropogenic factors. Many studies focused on the morphological and physical-chemical investigation of the archaeological remains found as well as the inorganic constituent of the soil. The organic fraction has been only roughly investigated and studies carried out on a molecular level are very limited. However, a more refined investigation of the chemical constituents of these soils can potentially reveal pivotal insights on the archaeological context such as past domestic habits and/or urban organization. It has been demonstrated that, among organic matter constituents, fecal steroids are promising biomarkers to track fecal input in many environmental matrixes such as water, soils, and sediments. In this paper we determine the fecal sterols and stanols content in dark homogeneous anthropogenic deposits and soils found in seven archaeological sites in Italy (from Verona, Como, Florence, Mel and Conegliano) dated from the Late Bronze Age and the Medieval Period, aiming to assess the major contribution of the fecal input using diagnostic fecal indexes obtained from specific fecal sterol proportions. To this purpose, a baseline was established by identifying the fecal fingerprint of domestic herbivores (caprids, ovines, equines and bovines) and omnivores (pigs). Based on their fecal fingerprint, we classified the deposits of seven studied sites into four distinct categories: i) the medieval sites of Verona, presented a strong mixed fecal input from both herbivores and humans; ii) the medieval sites of Como and Florence which showed a similar pattern, with a predominance of human fecal input over livestock; iii) the late medieval soils of Mel and the late bronze age Cultural Layers of Castellar di Leppia (Verona) which exhibited a generally scarce fecal contamination while iv) the final bronze age site of Conegliano (Treviso), a strong fecal contamination from herbivores was detected. These results thus demonstrate that for the Medieval Dark Earth the socio-cultural processes leading to the formation of the Dark Earth differ in terms of sewage and waste management and/or in animal husbandry practices.