FIGURE 5 from Single-Cell CD4 and CD8 T-Cell Secretome Profiling Reveals Temporal and Niche Differences in Acute Myeloid Leukemia Following Immune Checkpoint Blockade Therapy

Single-cell cytokine and polyfunctional group analysis at baseline. Heat map of cytokine expression by cluster for A. CD4 T cells. Annotation bars show cluster breakdown by sample location and timepoint. Hierarchical clustering was used to create new polyfunctional groups (CD4-G1-5), which are highlighted by black boxes. B, Boxplots for CD4 T cells show the comparison of complete responders (CR, brown) and nonresponders (NR, blue) at baseline by sample location in these newly defined polyfunctional groups for T cells. C, Heat map of cytokine expression by cluster for CD8 T cells with hierarchical clustering creating new polyfunctional groups (CD8-G1-6). D, Boxplots for CD8 T cells show comparison of CR (brown) and NR (blue) at baseline.