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To investigate the effect of the restoring effects of allicin (diallylthiosulfinate, DADS) on the altered lipid profile, osmotic fragility and membrane-bound enzymes in 7,12-dimethylbenz(a)anthracene (DMBA) -induced hamster buccal pouch carcinogenesis. 1 week after receiving DADS (20 mg/kg body weight) orally, the buccal pouches of hamsters were painted daily with 0.5% DMBA in liquid paraffin for 14 weeks.The experiment was terminated at the end of 16 weeks.The restoring effects of DADS were evaluated by measuring the lipid profile, osmotic fragility and membrane-bound enzymes were analyzed by using specific colorimetric methods.The modified levels of lipid profiles (total cholesterol, triglycerides, phospholipids, HDL, LDL and VLDL) in plasma, RBC-membrane and buccal mucosa tissues in DMBA-administered hamsters were normalized in DADS-treated animals.We observed an altered activity of erythrocyte membrane-bound enzyme (Na + K + -ATPase) and disturbed extracellular Na + and intracellular K + cation in the plasma tumor bearing animals, which suggests that the membrane permeability is affected during DMBA-induced oral carcinogenesis.The membrane stabilizing effects of DADS were confirmed by erythrocytes' osmotic fragility and the levels of sodium, potassium and the activity of Na + K + -ATPase were restored in DMBA-treated animals after treatment with the DADS.Increased erythrocyte fragility and permeability in cancer animals are probably due to their altered lipids, osmotic fragility and membrane-bound enzymes.Oral administration of DADS to cancer animals prevented the alterations in red cell fragility and the activity of membrane-bound Na + K + -ATPase, which indicates the role of DADS in maintaining the structural integrity of erythrocytes during carcinogenesis.
A Fri, study studied this question.