High-speed simultaneous label-free autofluorescence-multiharmonic (hSLAM) microscopy for multicellular dynamics

Label-free nonlinear microscopy allows for high-resolution and three-dimensional imaging of live biological specimens without the need for exogenous labels. The integration of multiple modalities further enhances molecular specificity and visualization diversity for metabolic and structural mapping of heterogeneous tissue architectures. In this work, we introduce high-speed simultaneous label-free autofluorescence-multiharmonic (hSLAM) microscopy, where a high-peak-power adaptive fiber source based on multimode fiber (MMF) is employed with a nonlinear fiber piano. We will also talk about how the higher speed SLAM enables multicellular dynamics in living tissues with higher spectral flexibility and peak power, providing new possibilities for bioimaging.