Multispectral fluorescence lifetime imaging microscope based on computational imaging techniques

Multispectral fluorescence lifetime imaging microscopy (λFLIM) is a powerful optical technique to investigate biological processes, which generally requires long acquisition time. Single Pixel Camera (SPC) is an imaging architecture base on Compressive Sensing (CS) techniques which allows to strongly reduce the acquisition time while preserving the information content at the cost of an increased computational time. In this work we present a λFLIM microscope based on CS-SPC architecture. We have tested the multiscale capability of the system by merging SPC zooming with data fusion and proposed a fast fitting framework, which runs in parallel with the acquisition, allowing a fast visualization.