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Open AccessCCS ChemistryRESEARCH ARTICLES16 Mar 2024Mass spectrometry Imaging Reveals the Redox and Metabolic Heterogeneity Jing Han, Huihui Liu, Yafeng Li, Junyu Chen, Ke Jia, Lingwei Meng, Caiqiao Xiong, Suming Chen and Zongxiu Nie Jing Han , Huihui Liu , Yafeng Li , Junyu Chen , Ke Jia , Lingwei Meng , Caiqiao Xiong , Suming Chen and Zongxiu Nie https://doi.org/10.31635/ccschem.024.202404147 SectionsSupplemental MaterialAboutPDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareFacebookTwitterLinked InEmail Oxidative stress is associated with various diseases. Therapeutic strategies targeting redox balance regulations have been tested. However, the lack of knowledge about the heterogeneous metabolism and redox balance within different regions of tissues limits the development of redox-targeted therapies. In this study, mass spectrometry imaging was used to profile the redox and metabolic heterogeneity in response to oxidative stress induced by in situ synthesis of Au particles. Our findings showed that the kidney cortex exhibited better glutathione-related reducibility than medulla to maintain redox homeostasis. The IDH1 and G6PD pathways were primary contributors to NADPH regeneration, assisting in the conversion of oxidized glutathione to glutathione. In solid tumors, oxidative stress induced infiltration of tumor-associated macrophages and suppressed the proliferation of polymorphonuclear cells, which played a crucial role in maintaining tumor redox homeostasis. Our study provided a new strategy to investigate the spatial metabolomic rewiring in tissues during oxidative challenge. Download figure Download PowerPoint Previous articleNext article FiguresReferencesRelatedDetails Issue AssignmentNot Yet AssignedSupporting Information Copyright & Permissions© 2024 Chinese Chemical Society Downloaded 0 times PDF downloadLoading ...
Han et al. (Sat,) studied this question.