Abstract 5502: Multiplex data utilizing a single-step staining and imaging workflow for the investigation of multiple sample types

Abstract Background: Tissue consists of heterogenous cell types, each with diverse functions and functional states, where spatial organization can impact patient health status. Resolving this complexity at the subcellular level has historically been challenged by image resolution, the number of targets that can be simultaneously assessed, and throughput. Such barriers can be broken using high plex and whole slide biomarker imaging data, in one single cycle thus also mitigating issues that arise with multiple cyclic rounds. Methods: A range of whole slide healthy and diseased tissue types were stained with 15 to 18-plex validated biomarker panels. The tissues’ inherent autofluorescence was isolated into discrete fluorescence channels for enhanced information about each tissue’s morphology. Whole slide spatial staining and imaging was conducted on the Orion™ spatial biology platform, and H Part 1 (Regular Abstracts) ; 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84 (6Suppl): Abstract nr 5502.