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Abstract A sensitive and rapid ultra-performance liquid chromatography coupled with -tandem mass spectrometry (UPLC-MS/MS) method was developed and validated to determine ceftibuten (CTB) and sulbactam (SUL) in human plasma. An ACQUITY UPLC HSS T3 C18 (2.1 × 100 mm), 1.8 μm column with gradient elution of water (0.1% formic acid) and acetonitrile was used for separation at a flow rate of 0.2 mL/min. This method involves a simple sample preparation with acetonitrile. The calibration curves of CTB and SUL in plasma showed good linearity over the concentration range of 0.50–25 μg/mL and with a correlation coefficient (r2) 0.99. This method was validated in terms of selectivity, linearity, precision, accuracy and stability. High precision was obtained with coefficients of variation 15%. Excellent recovery in the range of 90–104% was achieved for CTB and SUL was 86–110%. The method has the potential utility to support pharmacometric modeling in clinical practice and biopharmaceutic studies.
Valero-Rivera et al. (Sat,) studied this question.