FIGURE 3 from Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate

VCAN expression in different phenotypes and areas. IHC staining for VCAN completed on 26 tissues. Phenotypes for tissues based on CD8+ T-cell analysis. Images analyzed for VCAN percentage in whole area (A), each region (B), in areas split by phenotype (C) and in each phenotype split by zone (D). One-way ANOVA. E, Percentage of CD8+ T cells compared with percentage of VCAN in each zone. Phenotypes indicated by color of dot. Spearman rank correlation. F, Number of VCAN+ cells determined from RNAscope. Comparison between phenotypes in all areas and in specific zones. G, Versikine levels determined from IHC staining on 30 tissues. Comparison between phenotypes in all areas and in specific zones. H, VCAN stained on a consecutive slide. Comparison of VCAN and versikine in the different zones. Spearman rank correlation completed for each phenotype. *, P P