Chiral resolution of cationic piperazine derivatives by capillary electrophoresis using sulfated β‐cyclodextrin

Abstract This research focuses on the development and validation of a capillary electrophoresis (CE) method for the chiral separation of three H1‐antihistamine drugs chlorcyclizine, norchlorcyclizine, and neobenodine using sulfated β‐cyclodextrin (S‐β‐CD) as the chiral selector. The study explores various factors influencing the separation efficiency, including CD concentration, organic modifier content, voltage application, and buffer pH. Optimal conditions were identified as a 100 mM phosphate buffer (pH 6.0) with 34 mg mL –1 S‐β‐CD and 40% (v/v) methanol. The method demonstrated excellent linearity in calibration curves, with coefficients of determination exceeding 0.99 for each enantiomer. Precision studies revealed good intra‐ and inter‐day precision for migration times and peak areas. The limits of detection and quantification for the analytes were within the ranges of 5.9–11.4 and 18–34.6 µmol L –1 , respectively. Overall, the developed CE method offers a robust and precise approach for the chiral separation of H1‐antihistamine drugs, holding promise for pharmaceutical applications.