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Abstract Microwells array-based cellular self-assembly approaches are frequently utilized in organoid manufacturing due to their ability to generate consistent 3D cell spheroids. However, in the context of the microenvironments contained, multiple organ combinations and other complex organoid biofabrication processes are essential, and the lack of controllable inter- and intra-wells heterogeneity has hindered the broader application of these approaches. In this study, we introduce a micromanipulation-based robotic platform that integrates advanced robotic technology and visual-servo systems to enable the patterned and complex organoid biofabrication. By addressing cell seeding-induced fluid perturbations within microwells, we effectively control the spatial distribution of cell, promote self-assembly and achieve precisely arranged of varied types tissue spheroids. This platform supports advancements in complex organoid-based biomechanism discovery, drug screening, and other organoid-based investigations.
Gao et al. (Tue,) studied this question.